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Innovagen AB
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ActivX Inc
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Rabbit polyclonal to Nephrin. Conjugation note: Unconjugated Application note: IHC-p, ELISA Reactivity note: Human, Mouse, Rat
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Rabbit IgG polyclonal antibody for Nephrin NPHS1 detection Tested with WB in Human Mouse Rat
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Image Search Results
Journal: Nature protocols
Article Title: Directed differentiation of human induced pluripotent stem cells into mature kidney podocytes and establishment of a Glomerulus Chip
doi: 10.1038/s41596-018-0007-8
Figure Lengend Snippet: Immunofluorescence staining and scanning electron micrograph of cells during the differentiation process. Human iPS cell-derived mesoderm cells expressing hand1, goosecoid, and brachyury (a), intermediate mesoderm cells expressing WT1 and Pax2 markers (b), and podocytes expressing lineage characterization markers nephrin, WT1 and podocin, as well as the associated protein ApoL1 (c). (d) High magnification image of iPS cell-derived podocytes showing the development of foot processes that are positive for podocin. (e) Scanning electron micrograph of human iPS-derived podocytes showing the development of primary and secondary foot processes. Figures d, e modified with permission from Reference 5. Scale bars, (a-c) 100 μm, (d) 25 μm, (e) 2 μm.
Article Snippet: All-trans retinoic acid, 500 mg (Stem Cell Technologies, cat. no. 72262) Heat inactivated fetal bovine serum (FBS) (Thermo/Life Technologies, cat. no. 10082147) Penicillin-Streptomycin, liquid (100X) (Thermo/Life Technologies, cat. no. 15140–163) Antibodies and fluorescent stains Oct4 (R&D Systems, cat. no. AF1759) TRA-1–60 (Abcam, cat. no. ab16288) Goosecoid (R&D Systems, cat. no. AF4086) Brachyury (Abcam, cat. no. ab20680) HAND1 (Abcam, cat. no. ab196622) Pax2 (Invitrogen, cat. no. 71–6000) OSR1 (Novus Biologicals, cat. no. H00130497-M04 WT1 (Millipore, cat. no. MAB4234) Nephrin (Progen, cat. no. GP-N2) Podocin (Abcam, cat. no. ab50339) VE-cadherin (Santa Cruz Biotech, cat. no. sc-9989) Collagen type IV (Abcam, cat. no. ab6586) Integrin β1 (Abcam, cat. no. ab24693) ApoL1, anti-apolipoprotein L1 (Abcam, cat. no. ab85795) 4′,6-diamidino-2-phenylindole (DAPI) (Thermo/Life Technologies, cat. no. D1306) Oct4 conjugated to phycoerythrin (Oct4-PE) (Stem Cell Technologies, cat. no. 60093PE) Alexa Fluor 488 and Alexa Fluor 594-conjucated secondary antibodies ((Thermo/Life Technologies) WTI conjugated to APC (WT1-APC) (LifeSpan Biosciences, cat. no. LS-C224662/5744)
Techniques: Immunofluorescence, Staining, Derivative Assay, Expressing, Modification